Human beta -TrCP1/BTRC Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.
  • Source
    Monoclonal Mouse IgG1 Clone # 763524
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human beta -TrCP1 isoform 2
    Met1-Leu120
    Accession # Q9Y297-2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse beta -TrCP1/BTRC by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -TrCP1/BTRC at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
beta ‑TrCP1/BTRC in A172 Human Cell Line. beta ‑TrCP1/BTRC was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human beta ‑TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human beta -TrCP1/BTRC by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for beta -TrCP1/BTRC at approximately 84 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-TrCP1/BTRC
beta -TrCP1, also called BTRC ( beta -transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta -TrCP1 can form homodimers and heterodimers with beta -TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta -TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta -TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta -TrCP1, respectively.
  • Long Name:
    Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase
  • Entrez Gene IDs:
    8945 (Human); 12234 (Mouse); 361765 (Rat)
  • Alternate Names:
    beta-transducin repeat containing; betaTrCP; BETA-TRCP; betaTrCP1; beta-TrCP1; BTRC; BTRCP; bTrCP1; E3RSIkappaB; F-box and WD repeats protein beta-TrCP; F-box/WD repeat-containing protein 1A; FBW1A; FBXW1; FBXW1A; FBXW1ABTRCP; FWD1; MGC4643; pIkappaBalpha-E3 receptor subunit
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