Human BMP-1/PCP Antibody

Catalog # Availability Size / Price Qty
MAB1927
MAB1927-SP
Detection of Human BMP‑1/PCP by Western Blot.
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Product Details
Citations (5)
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Human BMP-1/PCP Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-1/PCP in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 264822
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP
Ala121-Gln730
Accession # NP_001190
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BMP-1/PCP antibody by Western Blot. View Larger

Detection of Human BMP‑1/PCP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human skin tissue. PVDF Membrane was probed with 2 µg/mL of Human BMP-1/PCP Monoclonal Antibody (Catalog # MAB1927) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BMP-1/PCP at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-1/PCP

Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).

References
  1. Wozney, J.M. et al. (1988) Science 242:1528.
  2. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
  3. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
  4. Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
  5. Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
Long Name
Bone Morphogenetic Protein 1
Entrez Gene IDs
649 (Human); 12153 (Mouse); 83470 (Rat)
Alternate Names
BMP1; BMP-1; bone morphogenetic protein 1; EC 3.4.24; EC 3.4.24.19; FLJ44432; Mammalian tolloid protein; mTld; PCOLC; PCP; PCP2; procollagen C-endopeptidase; Procollagen C-proteinase; TLD

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Citations for Human BMP-1/PCP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. BMP-1 disrupts cell adhesion and enhances TGF-beta activation through cleavage of the matricellular protein thrombospondin-1
    Authors: Cyril Anastasi, Patricia Rousselle, Maya Talantikite, Agnès Tessier, Caroline Cluzel, Alice Bachmann et al.
    Science Signaling
  2. Bone morphogenetic protein 1 is expressed in porcine ovarian follicles and promotes oocyte maturation and early embryonic development
    Authors: Deshun Shi
    J. Vet. Med. Sci., 2016-11-25;0(0):.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  3. Identification of a potential cardiac antifibrotic mechanism of torasemide in patients with chronic heart failure.
    Authors: Lopez B, Gonzalez A, Beaumont J, Querejeta R, Larman M, Diez J
    J. Am. Coll. Cardiol., 2007-08-13;50(9):859-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery
    Authors: Estibaliz Castillero, Ziad A. Ali, Hirokazu Akashi, Nicholas Giangreco, Catherine Wang, Eric J. Stöhr et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  5. Upregulation of Bone Morphogenetic Protein-1/Mammalian Tolloid and Procollagen C-Proteinase Enhancer-1 in Corneal Scarring
    Authors: Francois Malecaze, Dawiyat Massoudi, Pierre Fournie, Cyrielle Tricoire, Myriam Cassagne, Marilyne Malbouyres et al.
    Investigative Ophthalmology & Visual Science

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