Detects human BMP-5 in direct ELISAs and Western blots. In direct ELISAs, 50% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, or -7 is observed.
Monoclonal Mouse IgG2B Clone # 149107
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human BMP‑5 Asn313-His454 Accession # P22003
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38. The Neutralization Dose (ND50) is typically 2‑10 µg/mL in the presence of 1.5 µg/mL Recombinant Human BMP‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by BMP‑5 and Neutralization by Human BMP‑5 Antibody. Recombinant Human BMP‑5 (Catalog # 615‑BMC) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑5 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑5 Monoclonal Antibody (Catalog # MAB7151). The ND50 is typically 2-10 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and
functionally related BMPs which are members of the transforming growth
factor beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino
acid (aa) precursor protein that is cleaved at the dibasic cleavage site
(RxxR) to release the 20 kDa C-terminal mature protein (2). Mature
BMP-5 contains seven conserved cysteine residues involved in formation
of the cysteine knot and the single interchain disulfide bond.
Biologically active BMP-5 is a disulfide-linked homodimer of the
C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence
identity with mouse and rat BMP-5. Cellular responses to BMP-5 are
mediated by the formation of hetero-oligomeric complexes of type I and
type II serine/threonine kinase receptors (1). BMP-5 is expressed by
chondrocytes in proliferating and hypertrophic zones of bone growth
plates (3). It contributes to limb development by promoting
proliferation and differentiation of chondrocytes as well as apoptosis
of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which
cause C-terminal truncation or loss of the proteolytic cleavage site
result in multiple skeletal abnormalities, including the short ear
phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa
cells where it functions as an autocrine factor to promote GC
proliferation and inhibit their production of progesterone (7). In the
nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic
neuronal differentiation (8, 9). It is upregulated in oral squamous
carcinoma cells and induces the apoptosis of some myeloma cell lines
Chen, D. et al. (2004) Growth Factors 22:233.
Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
King, J.A. et al. (1994) Dev. Biol. 166:112.
Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
Jin, Y. et al. (2001) Oral Oncol. 37:225.
Ro, T.B. et al. (2004) Oncogene 23:3024.
Bone Morphogenetic Protein 5
Entrez Gene IDs:
653 (Human); 12160 (Mouse)
BMP5; BMP-5; bone morphogenetic protein 5; MGC34244
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