Intracellular Staining by Flow Cytometry
|Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c‑Myc Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC3696R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.|
Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH, and leucine zipper domains. The 439 aa human c-Myc has one potential O-glycosylation site plus three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shares 92% aa identity with mouse and rat c-Myc.
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