Human c-Myc APC-conjugated Antibody Summary
Accession # P01106
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc APC-conjugated Monoclonal Antibody (Catalog # IC3696A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.
Citation for Human c-Myc APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A novel luciferase-based assay for the detection of Chimeric Antigen Receptors
Authors: R Gopalakris, H Matta, S Choi, V Natarajan, R Prins, S Gong, A Zenunovic, N Narasappa, F Patel, R Prakash, V Chaudhary, V Sikri, SD Chitnis, A Kochegarov, D Wang, M Falat, M Kahn, PS Keerthipat, N Sharma, J Lenka, TM Stieben, J Braun, A Batra, K Purvis, K Ito, JH Lee, A Jeronimo, HM Zamora, A Membreno, Q Qiu, S Peshin, L Namburu, PM Chaudhary
Sci Rep, 2019;9(1):1957.
Sample Types: Whole Cells
Applications: Flow Cytometry
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