Human c-Myc APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products

Human c-Myc APC-conjugated Antibody Summary

Species Reactivity
Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
Monoclonal Mouse IgG1 Clone # 9E10
Protein A or G purified from hybridoma culture supernatant
C-terminal region peptide of human c-Myc
Accession # P01106
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of c‑Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc APC-conjugated Monoclonal Antibody (Catalog # IC3696A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.


Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Entrez Gene IDs
4609 (Human); 17869 (Mouse); 24577 (Rat)
Alternate Names
avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)

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Citation for Human c-Myc APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel luciferase-based assay for the detection of Chimeric Antigen Receptors
    Authors: R Gopalakris, H Matta, S Choi, V Natarajan, R Prins, S Gong, A Zenunovic, N Narasappa, F Patel, R Prakash, V Chaudhary, V Sikri, SD Chitnis, A Kochegarov, D Wang, M Falat, M Kahn, PS Keerthipat, N Sharma, J Lenka, TM Stieben, J Braun, A Batra, K Purvis, K Ito, JH Lee, A Jeronimo, HM Zamora, A Membreno, Q Qiu, S Peshin, L Namburu, PM Chaudhary
    Sci Rep, 2019;9(1):1957.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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