Human c-Myc Antibody

Catalog # Availability Size / Price Qty
MAB3696
MAB3696-SP
Human c-Myc Antibody in Data
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Product Details
Citations (3)
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Reviews (1)

Human c-Myc Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 9E10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Fan H, et al. (1998) Biochem. Cell. Biol. 76:125.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
c‑Myc is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in c‑Myc knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human c‑Myc by Western Blot. View Larger

Detection of Human c‑Myc by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of c‑Myc-tagged Protein by Western Blot. View Larger

Detection of c‑Myc-tagged Protein by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected (-) or transfected with c-Myc-tagged recombinant mouse Wnt-3a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc-tagged recombinant mouse Wnt-3a at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc Mono-clonal Antibody (Catalog # MAB3696, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Immunocytochemistry c-Myc in HEK293 Human Cell Line Transfected with c-Myc-tagged Serotonin Receptor. View Larger

c-Myc in HEK293 Human Cell Line Transfected with c-Myc-tagged Serotonin Receptor. c-Myc was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with c-Myc-tagged Serotonin Receptor using Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry c‑Myc in Human Prostate. View Larger

c‑Myc in Human Prostate. c-Myc was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human c‑Myc by Simple Western<sup>TM</sup>. View Larger

Detection of Human c‑Myc by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 74 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human c‑Myc by Simple Western<sup>TM</sup>. View Larger

Detection of Human c‑Myc by Simple WesternTM. Simple Western lane view shows c-Myc-tagged recombinant human ANGPTL6, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 161 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c-Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c-Myc at approximately 52 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Entrez Gene IDs
4609 (Human); 17869 (Mouse); 24577 (Rat)
Alternate Names
avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)

Product Datasheets

Citations for Human c-Myc Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Critical relevance of genomic gains of PRL-3/EGFR/c-myc pathway genes in liver metastasis of colorectal cancer
    Authors: T Tanaka, T Kaida, K Yokoi, S Ishii, N Nishizawa, H Kawamata, H Katoh, T Sato, T Nakamura, M Watanabe, K Yamashita
    Oncol Lett, 2019;17(1):1257-1266.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. U1 Adaptors suppress the KRAS-MYC oncogenic axis in human pancreatic cancer xenografts
    Authors: AT Tsang, C Dudgeon, L Yi, X Yu, R Goraczniak, K Donohue, S Kogan, MA Brenneman, ES Ho, SI Gunderson, DR Carpizo
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The Binding of the Bone Morphogenetic Protein Antagonist Gremlin to Kidney Heparan Sulfate: Such Binding is not Essential for BMP Antagonism
    Authors: Arnold Junior Tatsinkam
    Int. J. Biochem. Cell Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human c-Myc Antibody

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Human c-Myc Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human