Human c-Myc Antibody

  (2 citations)
(1 Review)
    
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Product Details
Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 9E10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C-terminal region peptide of human c-Myc
    Ala408-Ala439
    Accession # P01106
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Fan H, et al. (1998) Biochem. Cell. Biol. 76:125.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human c‑Myc by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c‑Myc at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of c‑Myc-tagged Protein by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected (-) or transfected with c-Myc-tagged recombinant mouse Wnt-3a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc-tagged recombinant mouse Wnt-3a at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c‑Myc Mono­clonal Antibody (Catalog # MAB3696, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Immunocytochemistry
c-Myc in HEK293 Human Cell Line Transfected with c-Myc-tagged Serotonin Receptor. c‑Myc was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with c-Myc-tagged Serotonin Receptor using Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
c‑Myc in Human Prostate. c‑Myc was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

  • Long Name:
    v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
  • Entrez Gene IDs:
    4609 (Human); 17869 (Mouse); 24577 (Rat)
  • Alternate Names:
    avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. U1 Adaptors suppress the KRAS-MYC oncogenic axis in human pancreatic cancer xenografts
    Authors: AT Tsang, C Dudgeon, L Yi, X Yu, R Goraczniak, K Donohue, S Kogan, MA Brenneman, ES Ho, SI Gunderson, DR Carpizo
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. The Binding of the Bone Morphogenetic Protein Antagonist Gremlin to Kidney Heparan Sulfate: Such Binding is not Essential for BMP Antagonism
    Authors: Arnold Junior Tatsinkam
    Int. J. Biochem. Cell Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-P

FAQs

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Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human c-Myc Antibody MAB3696 Array MAB3696
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Very Good
 WB Human Anonymous 01/25/2018
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Western Blot Human c-Myc Antibody MAB3696
Western Blot: Human c-Myc Antibody [MAB3696].

Summary

Application Western Blot
Sample Tested HEK293 human embryonic kidney cell line
Species Human

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