Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Insect - Drosophila melanogaster

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 9E10
View all c-Myc Primary Antibodies »

Product Specifications

Immunogen

C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106

Specificity

Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human c‑Myc PE‑conjugated Antibody

Detection of c-Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PE-conjugated Monoclonal Antibody (Catalog # IC3696P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human c‑Myc PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol

Reviewed Applications

Read 2 reviews rated 4.5 using IC3696P in the following applications:

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Alternate Names

cMyc, Myc, Myc2, Niard, Nird

Entrez Gene IDs

4609 (Human); 17869 (Mouse); 24577 (Rat)

Gene Symbol

MYC

UniProt

P01106

Additional c-Myc Products

Product Documents for Human c‑Myc PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human c‑Myc PE‑conjugated Antibody

For research use only

Citations for Human c‑Myc PE‑conjugated Antibody

Customer Reviews for Human c‑Myc PE‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Nice bright signal
    Name: Saul Rodriguez Roque
    Application: Flow Cytometry
    Sample Tested: HL-60 and THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 04/07/2026
    G4-stabilization mediated changes in myeloid cell lines upon pyridostatin (PDS) were analyzed after 24h treatment versus vehicle and unstained control (UC). The histograms are were plotted over single living cells. (Unpublished data)
    I used it at a 1:200 / 1:400 dilution with the FOXP3 permeabilization kit, and also methanol having both good result
    Human c‑Myc PE‑conjugated Antibody IC3696P
  • Human c-Myc PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: bone marrow
    Species: bone marrow cells
    Verified Customer | Posted 07/05/2024
    Human c‑Myc PE‑conjugated Antibody IC3696P

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