Human C-Peptide Quantikine ELISA Kit

R&D Systems | Catalog # DICP00

R&D Systems
100% Guarantee

Key Product Details

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)

Sensitivity

2.88 pmol/L

Assay Range

28.9-1850 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
View all C-Peptide ELISA Kits »

Product Summary for Human C-Peptide Quantikine ELISA Kit

The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and synthetic human Insulin C-Peptide.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pmol/L) 129 436 845 127 437 861
Standard Deviation 5.77 14.3 13.2 12.4 33.0 65.3
CV% 4.5 3.3 1.6 9.8 7.6 7.6

Recovery for Human C-Peptide Quantikine ELISA Kit

The recovery of human Insulin C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 84-106
EDTA Plasma (n=4) 101 95-108
Heparin Plasma (n=4) 102 90-115
Serum (n=4) 103 96-109
Urine (n=4) 97 81-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Insulin C-Peptide were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human C-Peptide ELISA Linearity

Scientific Data Images for Human C-Peptide Quantikine ELISA Kit

Human C-Peptide ELISA Standard Curve

Human C-Peptide ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Peptide

Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.

Long Name

Proinsulin C-Peptide

Alternate Names

C-P, Insulin C-Peptide, Proinsulin C-Peptide

Entrez Gene IDs

3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)

Gene Symbol

INS

Additional C-Peptide Products

Product Documents for Human C-Peptide Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human C-Peptide Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human C-Peptide Quantikine ELISA Kit

Customer Reviews for Human C-Peptide Quantikine ELISA Kit (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human C-Peptide Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human C-Peptide Quantikine ELISA Kit
    Name: Helder Andre
    Sample Tested: Rabbit biological fluids
    Verified Customer | Posted 08/16/2019
    Appropriate to detect human c-peptide in rabbit samples. Tested to contaminate the standard curve with rabbit serum and anterior chamber fluid of the eye with neglectable interference as compared to vehicle (diluent provided by the kit).
    Human C-Peptide Quantikine ELISA Kit DICP00

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for Human C-Peptide Quantikine ELISA Kit (DICP00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits