Human C-Peptide Quantikine ELISA Kit
Human C-Peptide Quantikine ELISA Kit Summary
Product Summary
Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pmol/L) | 129 | 436 | 845 | 127 | 437 | 861 |
| Standard Deviation | 5.77 | 14.3 | 13.2 | 12.4 | 33 | 65.3 |
| CV% | 4.5 | 3.3 | 1.6 | 9.8 | 7.6 | 7.6 |
Recovery
The recovery of human Insulin C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 84-106 |
| EDTA Plasma (n=4) | 101 | 95-108 |
| Heparin Plasma (n=4) | 102 | 90-115 |
| Serum (n=4) | 103 | 96-109 |
| Urine (n=4) | 97 | 81-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: C-Peptide
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human C-Peptide Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Investigation of the levels of circulating miR-29a, miR-122, sestrin 2 and inflammatory markers in obese children with/without type 2 diabetes: a case control study
Authors: KM Mohany, O Al Rugaie, O Al-Wutayd, A Al-Nafeesa
BMC endocrine disorders, 2021;21(1):152.
Species: Human
Sample Types: Serum
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A map of metabolic phenotypes in patients with myalgic encephalomyelitis/chronic fatigue syndrome
Authors: F Hoel, A Hoel, IK Pettersen, IG Rekeland, K Risa, K Alme, K Sørland, A Fosså, K Lien, I Herder, HL Thürmer, ME Gotaas, C Schäfer, RK Berge, K Sommerfelt, HP Marti, O Dahl, O Mella, Ø Fluge, KJ Tronstad
JCI Insight, 2021;6(16):.
Species: Human
Sample Types: Serum
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Anti?glycolipid disorder effect of epigallocatechin?3?gallate on high?fat diet and STZ?induced T2DM in mice
Authors: Z Ren, Z Yang, Y Lu, R Zhang, H Yang
Mol Med Rep, 2020;21(6):2475-2483.
Species: Mouse
Sample Types: Serum
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Appropriate to detect human c-peptide in rabbit samples. Tested to contaminate the standard curve with rabbit serum and anterior chamber fluid of the eye with neglectable interference as compared to vehicle (diluent provided by the kit).
