Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human Calreticulin-2
Thr20-Leu384
Accession # Q96L12

Specificity

Detects human Calreticulin-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Calreticulin-2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Calreticulin‑2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calreticulin-2

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Alternate Names

CALR3, Calreticulin2, Crt2

Entrez Gene IDs

125972 (Human); 73316 (Mouse); 364529 (Rat)

Gene Symbol

CALR3

UniProt

Additional Calreticulin-2 Products

Product Documents for Human Calreticulin-2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Calreticulin-2 Biotinylated Antibody

For research use only

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