Human Calreticulin-2 Antibody

Catalog # Availability Size / Price Qty
AF2927
AF2927-SP
Detection of Calreticulin2 in MCF‑7 Human Cell Line by Flow Cytometry.
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Human Calreticulin-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calreticulin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Calreticulin-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Calreticulin-2
Thr20-Leu384
Accession # Q96L12
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Calreticulin‑2
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Calreticulin2 in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of Calreticulin2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Goat Anti-Human Calreticulin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2927, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calreticulin-2

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Entrez Gene IDs
125972 (Human); 73316 (Mouse); 364529 (Rat)
Alternate Names
CALR3; calreticulin 2; calreticulin 3; Calreticulin2; Calreticulin-2; calreticulin-3; cancer/testis antigen 93; Crt2; CRT2calreticulin-2; CT93; FLJ25355; MGC26577

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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