Human/Canine CD11b/Integrin alpha M Alexa Fluor® 647-conjugated Antibody

Catalog #: AF1699R Datasheet / COA / SDS

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AF1699R-100UG

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Human/Canine CD11b/Integrin alpha M Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Canine

Specificity

Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Integrin alpha M and recombinant human Integrin alpha E is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin Î±M
Phe17-Asn1105
Accession # NP_001139280

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD11b/Integrin alpha M

Human ITGAM (Integrin alpha M subunit) is a 127kDa (predicted) glycoprotein, a member of the Integrin family of proteins. The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The ECD of human ITGAM shares 75.8% aa sequence identity with canine ITGAM ECD.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6