Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody
Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody Summary
Pro59-Asp414
Accession # Q16790
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 PE-conjugated Monoclonal Antibody (Catalog # FAB2188P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.
- Pastorek, J. et al. (1994) Oncogene 9:2877.
- Opavsky, R. et al. (1996) Genomics 33:480.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
- Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Product Datasheets
Citations for Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Absent in melanoma 2 enhances anti-tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer
Authors: D Chai, D Qiu, Z Zhang, S Yuchen Shi, G Wang, L Fang, H Li, H Li, H Tian, J Zheng
J. Cell. Mol. Med., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Absent in melanoma 2 enhances anti-tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer
Authors: D Chai, D Qiu, Z Zhang, S Yuchen Shi, G Wang, L Fang, H Li, H Li, H Tian, J Zheng
J. Cell. Mol. Med., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Combining DNA vaccine and AIM2 in H1 nanoparticles exert anti-renal carcinoma effects via enhancing tumor-specific multi-functional CD8+ T cell responses
Authors: D Chai, H Shan, G Wang, Q Zhang, H Li, L Fang, J Song, N Liu, Q Zhang, H Yao, J Zheng
Mol. Cancer Ther., 2018;0(0):.
Species: Transgenic
Sample Types: Whole Cells
Applications: Flow Cytometry
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Average Rating: 4.5 (Based on 2 Reviews)
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