Human Carbonic Anhydrase IX Quantikine ELISA Kit

R&D Systems | Catalog # DCA900

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)

Sensitivity

4.39 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
View all Carbonic Anhydrase IX/CA9 ELISA Kits »

Product Summary for Human Carbonic Anhydrase IX Quantikine ELISA Kit

The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human CA9

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 166 338 673 166 333 647
Standard Deviation 6.36 7.19 20.5 10.5 21.6 39.7
CV% 3.8 2.1 3.0 6.3 6.5 6.1

Recovery for Human Carbonic Anhydrase IX Quantikine ELISA Kit

The recovery of CA9 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 110 103-114
Citrate Plasma (n=4) 96 89-111
EDTA Plasma (n=4) 99 90-110
Heparin Plasma (n=4) 97 88-112
Serum (n=4) 97 87-106
Urine (n=4) 101 89-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CA9 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Carbonic Anhydrase IX/CA9 ELISA Linearity

Scientific Data Images for Human Carbonic Anhydrase IX Quantikine ELISA Kit

Human Carbonic Anhydrase IX/CA9 ELISA Standard Curve

Human Carbonic Anhydrase IX/CA9 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Carbonic Anhydrase IX/CA9

Carbonic anhydrases catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Human members of the CAs are secreted (CA6), membrane-bound (CA4, 9 to 12 and 14), cytosolic (CA1 to 3, 7, 8 and 13) or mitochondrial (CA5A and 5B). Some CAs may serve as markers for tumors and hypoxia. A subset of CAs lack CA activity due to point mutations but retain esterase function.

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Gene Symbol

CA9

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Human Carbonic Anhydrase IX Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Carbonic Anhydrase IX Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for Human Carbonic Anhydrase IX Quantikine ELISA Kit

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  • Human Carbonic Anhydrase IX Quantikine ELISA Kit
    Name: Tanya Hundal
    Sample Tested: human breast implant-associated anaplastic large cell lymphoma, human breast implant seroma, Human blood serum and Human Plasma
    Verified Customer | Posted 12/02/2020
    Pubmed: 32414854
    Human Carbonic Anhydrase IX Quantikine ELISA Kit DCA900
  • Human Carbonic Anhydrase IX Quantikine ELISA Kit
    Name: Aleksandra Gruslova
    Sample Tested: Human plasma samples
    Verified Customer | Posted 02/21/2018
    Used 100 ul of Standard, control and plasma samples in duplicates.
    Human Carbonic Anhydrase IX Quantikine ELISA Kit DCA900

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Protocols

View specific protocols for Human Carbonic Anhydrase IX Quantikine ELISA Kit (DCA900):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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