Human Caspr1 Antibody

Catalog # Availability Size / Price Qty
AF7548
AF7548-SP
Caspr1 in Human Brain.
1 Image
Product Details
Citations (1)
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Human Caspr1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspr1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Caspr2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Caspr1
Trp20-Gly1280
Accession # P78357
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Caspr1 in Human Brain. View Larger

Caspr1 in Human Brain. Caspr1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Caspr1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7548) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to paranodal axonal regions in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr1

CASPR1 (Contactin-ASsociated PRotein-1; also Neurexin-4 and p190) is a 180-195 kDa member of the neurexin family of proteins. It is expressed on both axons and dendrites of neurons, and likely serves the critical function of directing the movement of low MW contactin, AMPA receptors, and Na+ plus K+ channels to appropriate sites on the cell membrane. This indicates participation in key processes such as myelination and excitatory neurotransmission. Mature human CASRP1 is a 1365 amino acid (aa) type I transmembrane protein. It contains a 1264 aa extracellular region (aa 20-1283) plus an 80 aa cytoplasmic domain. The extracellular region is highly modular, possessing one discoidin (carbohydrate-binding) domain (aa 24-158), four laminin G-like domains (aa 203-513, 813-956 and 1088-1250), two EGF-like domains (aa 540-577 and 957-996) and one fibrinogen C-terminal globular regions (aa 576-795). There is one potential alternative start site at Met507. Over aa 20-1280, human CASPR1 shares 94% aa sequence identity with mouse CASPR1.

Long Name
Contactin Associated Protein-like 1
Entrez Gene IDs
8506 (Human); 53321 (Mouse)
Alternate Names
Caspr; Caspr1; CASPRcaspr1; CNTNAP; CNTNAP1; contactin associated protein 1; neurexin 4; Neurexin IV; neurexin-4; NRXN4contactin-associated protein 1; p190

Product Datasheets

Citation for Human Caspr1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Phenotypic heterogeneity and plasticity of isocortical and hippocampal astrocytes in the human brain.
    Authors: Sosunov A, Wu X, Tsankova N, Guilfoyle E, McKhann G, Goldman J
    J Neurosci, 2014;34(6):2285-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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