Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 248718
View all Cathepsin S Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331 (predicted)
Accession # P25774

Specificity

Detects pro and mature forms of human Cathepsin S in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, D, L, L2/V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human Cathepsin S Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)

Reviewed Applications

Read 3 reviews rated 3.7 using MAB1183 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

References

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Alternate Names

CTSS

Entrez Gene IDs

1520 (Human)

Gene Symbol

CTSS

UniProt

P25774

Additional Cathepsin S Products

Product Documents for Human Cathepsin S Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cathepsin S Antibody

For research use only

Citations for Human Cathepsin S Antibody

Customer Reviews for Human Cathepsin S Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
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  • Human Cathepsin S Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human Cathepsin S Antibody MAB1183
  • Human Cathepsin S Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/09/2019
  • Human Cathepsin S Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018

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