Human Cathepsin S Antibody

Catalog # Availability Size / Price Qty
MAB1183
MAB1183-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human Cathepsin S Antibody Summary

Species Reactivity
Human
Specificity
Detects pro and mature forms of human Cathepsin S in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, D, L, L2/V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.
Source
Monoclonal Rat IgG2B Clone # 248718
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331 (predicted)
Accession # P25774
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cathepsin S (Catalog # 1183-CY)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

References
  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Entrez Gene IDs
1520 (Human)
Alternate Names
Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886

Product Datasheets

Citation for Human Cathepsin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi-A Comparison of Analytical Properties of Silver-Gold and Pure Gold Chips
    Authors: P Falkowski, P Mrozek, Z Lukaszewsk, L Oldak, E Gorodkiewi
    Biosensors, 2021;11(9):.
    Species: Human
    Sample Types: Plasma
    Applications: Surface Plasmon Resonance

FAQs

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Reviews for Human Cathepsin S Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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3 Star
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Human Cathepsin S Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Cathepsin S Antibody
By Anonymous on 01/09/2019
Application: Microarray Sample Tested: EDTA Plasma Species: Human

Human Cathepsin S Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse