Human CCL17/TARC Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF364
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Human CCL17/TARC Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL17/TARC in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant mouse TARC, recombinant human (rh) MIP-1 alpha, rhMIP-1 beta, rhRANTES, rhMCP‑1, rhTECK, and rh6Ckine is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
Ala24-Ser94
Accession # Q92583
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL17/TARC (Catalog # 364-DN)

Human CCL17/TARC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL17/TARC Antibody (Catalog # MAB364)

Standard: Recombinant Human CCL17/TARC Protein (Catalog # 364-DN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

References
  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.
Entrez Gene IDs
6361 (Human); 20295 (Mouse)
Alternate Names
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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Citations for Human CCL17/TARC Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Sublingual immunotherapy alters salivary IgA and systemic immune mediators in timothy allergic children
    Authors: Johanna Huoman, Georgia Papapavlou, Anna Pap, Johan Alm, Lennart J. Nilsson, Maria C. Jenmalm
    Pediatric Allergy and Immunology
  2. Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation
    Authors: Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén
    Pediatric Research
  3. Effect of the topical application of an ethanol extract of quince seeds on the development of atopic dermatitis-like symptoms in NC/Nga mice
    Authors: T Kawahara, K Tsutsui, E Nakanishi, T Inoue, Y Hamauzu
    BMC Complement Altern Med, 2017-01-31;17(1):80.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Lung transplantation affects expression of the chemokine receptor type 4 on specific T cell subsets.
    Authors: Paantjens AW, van de Graaf EA, Kwakkel-van Erp JM, Hoefnagel T, van Kessel DA, van den Bosch JM, Otten HG
    Clin. Exp. Immunol., 2011-10-01;166(1):103-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007-10-01;179(7):4840-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  7. Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
    Authors: Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN
    J. Immunol., 2001-06-01;166(11):6899-906.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  8. Placental immune response to apple allergen in allergic mothers
    Authors: Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al.
    Journal of Reproductive Immunology
  9. A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization
    Authors: T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm
    Clinical & Experimental Allergy

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Reviews for Human CCL17/TARC Biotinylated Antibody

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Human CCL17/TARC Biotinylated Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Cell culture supernatant,Serum and Plasma Species: Human

The TARC ELISA we made used MAB364 as the capture and BAF364 as the detection with recombinant antige 364-DN as the immunoassay standard.