Human CCL20/MIP-3 alpha Antibody

Catalog # Availability Size / Price Qty
MAB360-SP
MAB360-100
MAB360-500
Detection of Recombinant Human CCL20/MIP‑3 alpha  by Western Blot.
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Product Details
Citations (45)
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Human CCL20/MIP-3 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL19, rhCXCL1, rhCCL16, rhCCL11, recombinant mouse CCL20/MIP-3 alpha, or recombinant rat CCL20/MIP-3 alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 67310
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Human CCL20/MIP-3 alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog # BAF360)

Standard: Recombinant Human CCL20/MIP-3 alpha Protein (Catalog # 360-MP)

Neutralization
Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 0.2-2 µg/mL in the presence of 1 ng/mL Recombinant Human CCL20/MIP‑3 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human CCL20/MIP-3a antibody by Western Blot. View Larger

Detection of Recombinant Human CCL20/MIP‑3 alpha by Western Blot. Western blot shows 100 ng of Recombinant Human CCL20/MIP-3a (Catalog # 360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog # 760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization View Larger

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP-3a (Catalog # 360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360). The ND50 is typically 0.2-2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Human CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6.
    Authors: Gruol DL, Puro A, Hao C et al.
    J Neuroimmunol.
  2. FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma
    Authors: Y Wang, Z Lyu, Y Qin, X Wang, L Sun, Y Zhang, L Gong, S Wu, S Han, Y Tang, Y Jia, DL Kwong, N Kam, XY Guan
    Theranostics, 2020-09-16;10(25):11535-11548.
  3. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018-08-13;15(1):226.
  4. Xenotransplantation of adult hippocampal neural progenitors into the developing zebrafish for assessment of stem cell plasticity
    Authors: EJ Sandquist, JJ Essner, DS Sakaguchi
    PLoS ONE, 2018-05-24;13(5):e0198025.
  5. Head-to-Head Study of Developmental Neurotoxicity and Resultant Phenotype in Rats: alpha-Hexabromocyclododecane versus Valproic Acid, a Recognized Model of Reference for Autism Spectrum Disorders
    Authors: C Morel, A Christophe, K Maguin-Gat, J Paoli, JD Turner, H Schroeder, N Grova
    Toxics, 2022-04-06;10(4):.
  6. Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20–CCR6 axis
    Authors: Hiroshi Kano, Kouji Izumi, Kaoru Hiratsuka, Ren Toriumi, Ryunosuke Nakagawa, Shuhei Aoyama et al.
    Cancer Science
  7. Deficiency of Yes-Associated Protein Induces Cataract in Mice
    Authors: He Q, Gao Y, Wang, T et al.
    Aging Dis.
  8. Changes in arginase isoforms in a murine model of neonatal brain hypoxia-ischemia.
    Authors: Mike, J K, Pathipati, P Et al.
    Pediatr Res
  9. Ropinirole Cotreatment Prevents Perivascular Glial Recruitment in a Rat Model of L-DOPA-Induced Dyskinesia
    Authors: Elabi, OF;Espa, E;Skovgård, K;Fanni, S;Cenci, MA;
    Cells
  10. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Norepinephrine activates ?(1) -adrenergic receptors at the inner nuclear membrane in astrocytes
    Authors: Benton KC, Wheeler DS, Kurtoglu B et al.
    Glia
  12. Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium
    Authors: P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger
    Cell Reports, 2022-03-29;38(13):110571.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Microglial ablation does not affect opioid-induced hyperalgesia in rodents
    Authors: Liu X, Liu BL, Yang Q et al.
    Pain
  14. Inhibition of Gasdermin D-Mediated Pyroptosis Attenuates the Severity of Seizures and Astroglial Damage in Kainic Acid-Induced Epileptic Mice
    Authors: Xia L, Liu L, Cai Y et al.
    Frontiers in Pharmacology
  15. Elamipretide (SS-31) Improves Functional Connectivity in Hippocampus and Other Related Regions Following Prolonged Neuroinflammation Induced by Lipopolysaccharide in Aged Rats
    Authors: Yang Liu, Huiqun Fu, Yan Wu, Binbin Nie, Fangyan Liu, Tianlong Wang et al.
    Frontiers in Aging Neuroscience
  16. Overexpression of B7‑H4 promotes renal cell carcinoma progression by recruiting tumor‑associated neutrophils via upregulation of CXCL8
    Authors: Anqi Li, Ningyue Zhang, Zhiming Zhao, Yali Chen, Liang Zhang
    Oncology Letters
  17. Region-Specific Transcriptional Control of Astrocyte Function Oversees Local Circuit Activities
    Authors: Anna Yu-Szu Huang, Junsung Woo, Debosmita Sardar, Brittney Lozzi, Navish A Bosquez Bosquez Huerta, Chia-Ching John Lin et al.
    Neuron
  18. Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
    Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
    Mucosal Immunol, 2020-04-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. Recovery of Encapsulated Adult Neural Progenitor Cells from Microfluidic-Spun Hydrogel Fibers Enhances Proliferation and Neuronal Differentiation
    Authors: Bhavika B. Patel, Marilyn C. McNamara, Laura S. Pesquera-Colom, Emily M. Kozik, Jasmin Okuzonu, Nicole N. Hashemi et al.
    ACS Omega
  20. A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity
    Authors: Fadi Jacob, Ryan D. Salinas, Daniel Y. Zhang, Phuong T.T. Nguyen, Jordan G. Schnoll, Samuel Zheng Hao Wong et al.
    Cell
  21. Spinal Interleukin-1 beta Inhibits Astrocyte Cytochrome P450c17 Expression Which Controls the Development of Mechanical Allodynia in a Mouse Model of Neuropathic Pain
    Authors: Sheu-Ran Choi, Ho-Jae Han, Alvin J. Beitz, Jang-Hern Lee
    Frontiers in Molecular Neuroscience
  22. Inhibiting Bone Morphogenetic Protein 4 Type I Receptor Signaling Promotes Remyelination by Potentiating Oligodendrocyte Differentiation
    Authors: Alistair E. Govier-Cole, Rhiannon J. Wood, Jessica L. Fletcher, David G. Gonsalvez, Daniel Merlo, Holly S. Cate et al.
    eNeuro
  23. TGF beta -Signaling and FOXG1-Expression Are a Hallmark of Astrocyte Lineage Diversity in the Murine Ventral and Dorsal Forebrain
    Authors: Stefan Christopher Weise, Alejandro Villarreal, Stefanie Heidrich, Fariba Dehghanian, Christian Schachtrup, Sigrun Nestel et al.
    Frontiers in Cellular Neuroscience
  24. Cofilin-actin rod formation in neuronal processes after brain ischemia
    Authors: Seok Joon Won, Angela M. Minnella, Long Wu, Claire H. Eun, Eric Rome, Paco S. Herson et al.
    PLOS ONE
  25. Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm
    Authors: B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho
    Sci Rep, 2017-12-11;7(1):17331.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. CITED2 attenuates macrophage recruitment concordant with the downregulation of CCL20 in breast cancer cells
    Authors: Swaathi Jayaraman, Michele Doucet, Scott L. Kominsky
    Oncology Letters
  27. CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected individuals
    Authors: C. Loiseau, M. Requena, M. Mavigner, M. Cazabat, N. Carrere, B. Suc et al.
    Mucosal Immunology
  28. Disruption of CCL20-CCR6 interaction inhibits metastasis of advanced cutaneous T-cell lymphoma
    Authors: Sho Ikeda, Akihiro Kitadate, Mitsugu Ito, Fumito Abe, Miho Nara, Atsushi Watanabe et al.
    Oncotarget
  29. Dysregulation of astrocyte extracellular signaling in Costello syndrome
    Authors: Robert Krencik, Kenton C. Hokanson, Aditi R. Narayan, Jill Dvornik, Gemma E. Rooney, Katherine A. Rauen et al.
    Science Translational Medicine
  30. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
    Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
    Autophagy, 2013-12-04;10(2):257-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Evidence for NG2-glia Derived, Adult-Born Functional Neurons in the Hypothalamus
    Authors: Sarah C. Robins, Eric Trudel, Olivia Rotondi, Xiaohong Liu, Tina Djogo, Darya Kryzskaya et al.
    PLoS ONE
  32. Assessment of Adult Neurogenesis in Mice
    Authors: Yung‐Wei Pan, Wenbin Wang, Zhengui Xia
    Current Protocols in Toxicology
  33. Expression of nuclear factor one A and -B in the olfactory bulb
    Authors: Céline Plachez, Kathleen Cato, Robert C McLeay, Yee Hsieh Evelyn Heng, Timothy L Bailey, Richard M Gronostajski et al.
    The Journal of Comparative Neurology
  34. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011-11-02;17(24):7529-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  36. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
    Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
    J. Immunol., 2008-11-15;181(10):7420-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  37. Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
    Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
    Am. J. Pathol., 2008-05-23;173(1):195-204.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008-02-01;214(3):328-36.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  39. Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
    Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
    Clin. Exp. Immunol., 2007-10-09;150(3):567-75.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  40. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2006-12-29;109(9):3649-57.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  41. Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
    Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
    J. Immunol., 2006-08-01;177(3):1941-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  43. Astrocyte progenitor transplantation promotes regeneration of bulbospinal respiratory axons, recovery of diaphragm function, and a reduced macrophage response following cervical spinal cord injury
    Authors: Goulao M, Ghosh B, Urban MW et al.
    Glia
  44. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer
    Authors: Choi YW, Kim YH, Oh SY et al.
    Advanced Science
  45. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
    Authors: Hagiwara, Kunie, Obayashi, Takeshi, Sakayori, Nobuyuki, Yamanishi, Emiko, Hayashi, Ryuhei, Osumi, Noriko, Nakazawa, Toru, Nishida, Kohji
    PLoS ONE, 2014-01-20;9(1):e84072.

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Human CCL20/MIP-3 alpha Antibody
By Anonymous on 09/29/2021
Application: WB Sample Tested: Lymph node cells Species: Human