Human CCL20/MIP-3 alpha Antibody Summary
Ala27-Met96 (Asn95Asp)
Accession # P78556.1
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Applications
Human CCL20/MIP-3 alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Recombinant Human CCL20/MIP‑3 alpha by Western Blot. Western blot shows 100 ng of Recombinant Human CCL20/MIP-3a (Catalog # 360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog # 760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP-3a (Catalog # 360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360). The ND50 is typically 0.2-2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.
CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
- Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
- Hromas, R. et al. (1997) Blood 89:3315.
- Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Product Datasheets
Citations for Human CCL20/MIP-3 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium
Authors: P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger
Cell Reports, 2022;38(13):110571.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
Mucosal Immunol, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm
Authors: B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho
Sci Rep, 2017;7(1):17331.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
Autophagy, 2014;10(2):257-68.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clin. Cancer Res., 2011;17(24):7529-38.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
J. Pathol., 2008;214(3):328-36.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
J. Immunol., 2008;181(10):7420-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
Am. J. Pathol., 2008;173(1):195-204.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
Blood, 2007;109(9):3649-57.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
Clin. Exp. Immunol., 2007;150(3):567-75.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
J. Neuroimmunol., 2006;172(1):156-65.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
J. Immunol., 2006;177(3):1941-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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