Human CCL22/MDC Antibody
Human CCL22/MDC Antibody Summary
Gly25-Gln93
Accession # O00626.1
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Applications
Human CCL22/MDC Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody. Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL22/MDC Monoclonal Antibody (Catalog # MAB336). The ND50 is typically 0.6-3.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL22/MDC
CCL22, also named stimulated T cell chemotactic protein (STCP-1) and MDC, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.
- Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
- Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
- Pal, R. et al. (1997) Science 278:5338.
Product Datasheets
Citations for Human CCL22/MDC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
Cancers, 2021;13(6):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
p65/miR-23a/CCL22 axis regulated regulatory T cells recruitment in hepatitis B virus positive hepatocellular carcinoma
Authors: ZQ Li, HY Wang, QL Zeng, JY Yan, YS Hu, H Li, ZJ Yu
Cancer Med, 2019;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
CCL22 is a biomarker of cartilage injury and plays a functional role in chondrocyte apoptosis
Authors: G Ren, JL Whittaker, C Leonard, D De Rantere, DSJ Pang, P Salo, M Fritzler, M Kapoor, APJ de Koning, JL Jaremko, CA Emery, RJ Krawetz
Cytokine, 2019;115(0):32-44.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010;184(6):2886-98.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
Authors: Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
Int. J. Cancer, 2008;122(10):2286-93.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis.
Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B
Blood, 2007;111(2):761-6.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
J. Immunol., 2007;179(7):4840-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24.
Species: Human
Sample Types: BALF
Applications: ELISA Development -
Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.
Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping
Nat Med, 2004;10(9):942-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CCR4 blockade does not inhibit allergic airways inflammation.
Authors: Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I
J. Leukoc. Biol., 2003;74(4):558-63.
Species: Guinea Pig
Sample Types: BALF
Applications: ELISA Development
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Reviews for Human CCL22/MDC Antibody
Average Rating: 4 (Based on 2 Reviews)
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We used this antibody in an in-house ELISA along with pAb (AF336) and protein (336-MD-025) to detect CCL22 in human serum and plasma. This combination could detect CCL18 in human serum and plasma.
