Human CCL23/MPIF‑1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF371
Product Details
Citations (1)
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Human CCL23/MPIF‑1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL23/MPIF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 10% cross-reactivity with recombinant human (rh) CK beta 8-1 (a splice variant of rhMPIF-1) is observed, and less than 0.1% cross-reactivity with rhHCC-1, rhMIP-1δ, and rhMPIF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL23/MPIF-1 (R&D Systems, Catalog # 371-MP)
Arg22-Asn120
Accession # P55773
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP)

Human CCL23/MPIF-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL23/MPIF‑1 Antibody (Catalog # MAB3711)

Standard: Recombinant Human CCL23/MPIF-1 Protein (Catalog # 371-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL23/MPIF-1

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.

References
  1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
  2. Youn, B-S. et al. (1998) Blood 91:3118.
  3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
  4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.
Entrez Gene IDs
6368 (Human)
Alternate Names
CCL23; CCL23/MPIF-1; MPIF1; MPIF-1

Product Datasheets

Citation for Human CCL23/MPIF‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CCL23 expression is induced by IL-4 in a STAT6-dependent fashion.
    Authors: Novak H, Muller A, Harrer N, Gunther C, Carballido JM, Woisetschlager M
    J. Immunol., 2007;178(7):4335-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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