Human CCL8/MCP-2 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL8/MCP-2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.
  • Source
    Monoclonal Mouse IgG1 Clone # 35509
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL8/MCP-2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)
    • Human CCL8/MCP-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CCL8/MCP‑2 Antibody (Catalog # MAB281)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CCL8/MCP‑2 Biotinylated Antibody (Catalog # BAF281)
  • ELISA Standard
     
    Recombinant Human CCL8/MCP-2 Protein (Catalog # 281-CP)
  • Neutralization
    Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CCL8/MCP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody. Recombinant Human CCL8/MCP‑2 (Catalog #
281-CP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human
CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2
CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.
  • Entrez Gene IDs:
    6355 (Human); 20307 (Mouse)
  • Alternate Names:
    C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
    Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
    Genes Immun, 2016;17(1):52-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  2. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  3. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
    Authors: Struyf S, Proost P, Vandercappellen J
    Eur. J. Immunol., 2009;39(3):843-57.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
    Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
    Ann. Rheum. Dis., 2006;65(3):294-300.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
    Authors: Tay SS, McCormack A, Rose ML
    Transplantation, 2004;78(7):987-94.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

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ELISA Human CCL8/MCP-2 Antibody MAB281
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Very Good
 ELISA Human Anonymous 11/20/2017
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ELISA Human CCL8/MCP-2 Antibody MAB281
ELISA: Human CCL8/MCP-2 Antibody [MAB281]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis antibody was used as the capture in an ELISA with BAF281 as the detection antibody.

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