CCR5 in Human PBMCs. CCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB183) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR5 is a G protein-linked seven transmembrane domain chemokine
receptor. CCR5 serves as a receptor for several chemokines including
MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor
for Macrophage Tropic HIV-1 infection.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J Nature, 2013;493(7430):51-5. Species: Human Sample Type: whole cells Application: Bioassay