Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry.
Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM‑A/B APC‑conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.
Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
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