Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 152254
View all CCRL2/CRAM-A/B Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CRAM-A
Met1-Val356 (Val180Met)
Accession # NP_001124382

Specificity

Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCRL2/CRAM‑A/B APC‑conjugated Antibody

Detection of CCRL2/CRAM-A/B antibody in PBMCs antibody by Flow Cytometry.

Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry.

Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Detection of CCRL2/CRAM‑A/B in MDA-MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (IC0041A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CCRL2/CRAM‑A/B APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MDA-MB-231 human breast adenocarcinoma cell line or Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCRL2/CRAM-A/B

CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.

References

  1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
  2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
  3. Hartmann, T.N. et al. (2008) Immunology 125:252.
  4. Leick, M. et al. (2010) Immunology 129:536.

Long Name

CC Chemokine Receptor-like 2

Alternate Names

ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR

Entrez Gene IDs

9034 (Human)

Gene Symbol

CCRL2

UniProt

NP_001124382

Additional CCRL2/CRAM-A/B Products

Product Documents for Human CCRL2/CRAM‑A/B APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CCRL2/CRAM‑A/B APC‑conjugated Antibody

For research use only

Citations for Human CCRL2/CRAM‑A/B APC‑conjugated Antibody

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