Human CCRL2/CRAM-A/B APC-conjugated Antibody Summary
Accession # NP_001124382
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry. Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCRL2/CRAM‑A/B in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (IC0041A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.
- Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
- Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
- Hartmann, T.N. et al. (2008) Immunology 125:252.
- Leick, M. et al. (2010) Immunology 129:536.
Citation for Human CCRL2/CRAM-A/B APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Mesenchymal stromal cell-secreted chemerin is a novel immunomodulatory molecule driving the migration of ChemR23-expressing cells
Authors: Paola Vinci
Sample Types: Whole Cells
Applications: Flow Cytometry
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