Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 496920
View all CD109 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD109
Val22-Ser1268 (Tyr703Ser & Thr1241Met)
Accession # Q6YHK3

Specificity

Detects human CD109 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD109 Alexa Fluor® 488-conjugated Antibody

Detection of CD109 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD109 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 2 ug/mL PHA for 5 days were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and (A) Mouse Anti-Human CD109 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4385G) or (B) Alexa Fluor® 488-conjugated Mouse IgG2A isotype control (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD109 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human PBMC treated with PHA

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD109

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

References

  1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
  2. Lin, M. et al. (2002) Blood 99:1683.
  3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
  4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
  5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
  6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
  7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
  8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
  9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
  10. Solomon, K.R. et al. (2004) Gene 327:171.
  11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
  12. Schuh, A.C. et al. (2002) Blood 99:1692.
  13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
  14. Rozman, P. (2002) Transpl. Immunol. 10:165.

Alternate Names

CD109, CPAMD7

Entrez Gene IDs

135228 (Human); 235505 (Mouse); 363104 (Rat)

Gene Symbol

CD109

UniProt

Q6YHK3

Additional CD109 Products

Product Documents for Human CD109 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD109 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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