Human/Mouse CD109 PE-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human CD109 in direct ELISAs. Detects human and mouse CD109 in flow cytometry
  • Source
    Monoclonal Mouse IgG2A Clone # 496920
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD109
    Val22-Ser1268 (Tyr703Ser & Thr1241Met)
    Accession # Q6YHK3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD109 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse CD109 PE‑conjugated Monoclonal Antibody (Catalog # FAB4385P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD109 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human/Mouse CD109 PE‑conjugated Monoclonal Antibody (Catalog # FAB4385P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD109 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 2 ug/mL PHA for 5 days were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and (A) Mouse Anti-Human CD109 PE-conjugated Monoclonal Antibody (Catalog # FAB4385P) or (B) PE-conjugated Mouse IgG2A isotype control (Catalog # IC003P).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD109

CD109 is a GPI-anchored member of the alpha -2-Macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

  • References:
    1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
    2. Lin, M. et al. (2002) Blood 99:1683.
    3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
    4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
    5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
    6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
    7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
    8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
    9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
    10. Solomon, K.R. et al. (2004) Gene 327:171.
    11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
    12. Schuh, A.C. et al. (2002) Blood 99:1692.
    13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
    14. Rozman, P. (2002) Transpl. Immunol. 10:165.
  • Entrez Gene IDs:
    135228 (Human); 235505 (Mouse); 363104 (Rat)
  • Alternate Names:
    150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1
Related Research Areas
Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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