Human CD14 PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3832C-100
FAB3832C-025

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Detection of CD14 in Human PBMCs by Flow Cytometry.
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Citations (3)
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Human CD14 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD14 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 134620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD14 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB3832C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Citations for Human CD14 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Human Monocyte Subsets Are Transcriptionally and Functionally Altered in Aging in Response to Pattern Recognition Receptor Agonists
    Authors: TU Metcalf, PA Wilkinson, MJ Cameron, K Ghneim, C Chiang, AM Wertheimer, JB Hiscott, J Nikolich-Z, EK Haddad
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Global analyses revealed age-related alterations in innate immune responses after stimulation of pathogen recognition receptors.
    Authors: Metcalf T, Cubas R, Ghneim K, Cartwright M, Grevenynghe J, Richner J, Olagnier D, Wilkinson P, Cameron M, Park B, Hiscott J, Diamond M, Wertheimer A, Nikolich-Zugich J, Haddad E
    Aging Cell, 2015;14(3):421-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Immune activation alters cellular and humoral responses to yellow fever 17D vaccine.
    Authors: Muyanja E, Ssemaganda A, Ngauv P, Cubas R, Perrin H, Srinivasan D, Canderan G, Lawson B, Kopycinski J, Graham A, Rowe D, Smith M, Isern S, Michael S, Silvestri G, Vanderford T, Castro E, Pantaleo G, Singer J, Gillmour J, Kiwanuka N, Nanvubya A, Schmidt C, Birungi J, Cox J, Haddad E, Kaleebu P, Fast P, Sekaly R, Trautmann L, Gaucher D
    J Clin Invest, 2014;124(7):3147-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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