Human CD164 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5790N-100UG
Human CD164 Alexa Fluor® 700-conjugated Antibody in Flow Cytometry
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Human CD164 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD164 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD164 is observed.
Source
Monoclonal Mouse IgG3 Clone # 502021
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD14
Asp24-Leu197
Accession # Q04900
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD164 in CXCL12-treated PC-3 Human Cell Line by Flow Cytometry. View Larger

Detection of CD164 in CXCL12-treated PC-3 Human Cell Line by Flow Cytometry. PC-3 prostate cancer cell line treated with Recombinant Human CXCL12 (200ng/ml, Catalog # 6448-SD) for 2 hours (filled histogram), or PC-3 cell line untreated (open histogram) was stained with Mouse Anti-Human CD164 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB5790N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD164

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. CD164 was identified as a potential regulator of homing in a prostate cancer screening for CXCL12-responsive adhesion molecules (1). Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

References
  1. Havens, A.M. et al. (2006) BMC Cancer 2006, 6:195.
Entrez Gene IDs
8763 (Human); 53599 (Mouse)
Alternate Names
CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin

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