Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu202
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

Human CD20

Specificity

Stains hCD20-eGFP transfected HEK293 cells in flow cytometry. 

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Detection of CD20 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CD20-eGFP (A) vs irrelevant-GFP (B) were stained with Human Anti-Human CD20 Monoclonal Antibody (Catalog # MAB11595) followed by Allophycocyanin-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human CD20-eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD20

Ocrelizumab is an anti-human CD20 antibody used for the treatment of advanced or relapsing multiple sclerosis.  It is a humanized recombinant anti human CD20 antibody, and is used to specifically deplete B-cells.  A proposed pharmacological mechanism of action is that the antibody most likely binds to the B-cell surface CD20 resulting in antibody-dependent cellular cytotoxicity and complement-mediated cell lysis, but does not impact B-cell reconstitution and preexisting humoral immunity.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

For research use only

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