Human CD20 (Research Grade Tositumomab Biosimilar) Antibody

R&D Systems | Catalog # MAB10440

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu11A
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

Human CD20

Specificity

Detects Human CD20 based on Tositumomab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Tositumomab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD20 (Research Grade Tositumomab Biosimilar) Antibody

Detection of CD20 (Tositumomab) antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD20 (Tositumomab) in Human PBMCs by Flow Cytometry.

Human PBMCs were stained with (A) Human Anti-Human CD20 (Research Grade Tositumomab Biosimilar) Monoclonal Antibody (Catalog # MAB10440) or (B) unstained, followed by followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 (Research Grade Tositumomab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD20

Tositumomab is a biosimilar directed against CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for Human CD20 (Research Grade Tositumomab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD20 (Research Grade Tositumomab Biosimilar) Antibody

For research use only

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FAQs

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