Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI.
Monoclonal Mouse IgG1 Clone # 57703
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27 Thr21-Ile192 Accession # P26842
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CD27/TNFRSF7 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD27/TNFRSF7 APC‑conjugated Monoclonal Antibody (Catalog # FAB382A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human CD27 is a 50-55 kDa lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes as well as NK cells, B cells, and CD4+ and CD8+ T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4+ compartment, it is preferentially expressed on CD45RA+ cells. In contrast, it is preferentially expressed on CD45RO+ cells in the CD8+ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble 32 kDa protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 19 aa signal, a 173 aa extracellular domain, a 21 aa transmembrane domain, and a 48 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and multiple activated leukocytes including NK cells, CD4+ and CD8+ T cells, and gamma δ T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses. Over aa 21-192, human and mouse CD27 share 60% aa sequence identity.
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