Human CD36/SR-B3 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB19551N-100UG
Detection of CD36/SR‑B3 in HepG2 Human Cell Line by Flow Cytometry.
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Human CD36/SR-B3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD36/SR‑B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD36 is observed.
Source
Monoclonal Rat IgG2B Clone # 255606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36/SR‑B3
Gly30-Asn439
Accession # P16671
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD36/SR‑B3 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of CD36/SR‑B3 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rat Anti-Human CD36/SR‑B3  Alexa Fluor® 700‑conjugated  Monoclonal Antibody (Catalog # FAB19551N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells,
beta ‑amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References
  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
  3. Husemann, J. et al. (2002) Glia 40:195.
  4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
  5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Long Name
Scavenger Receptor Class B, Member 3
Entrez Gene IDs
948 (Human); 12491 (Mouse)
Alternate Names
CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

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