Human CD42b/GPIb  alpha Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4067T-100UG
Detection  of CD42b/GPIb  alpha  in Human CD41+ Platelets by  Flow Cytometry.
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Human CD42b/GPIb  alpha Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD42b/GPIb alpha  in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 486805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD42b/GPIb alpha
His17-Leu505
Accession # P07359
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CD42b/GPIb  alpha  in Human CD41+ Platelets by  Flow Cytometry. View Larger

Detection of CD42b/GPIb  alpha in Human CD41+ Platelets by Flow Cytometry. Human platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human CD42b/GPIb  alpha Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB4067T) or (B) Mouse IgG1 Alexa Fluor® 594 Isotype Control (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD42b/GPIb alpha

Platelet glycoprotein Ib alpha chain (GPIb  alpha ), also known as CD42b, is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human CD42b contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature CD42b are known to exist but have unknown significance (7). The ECD of human CD42b shares 48‑51% aa identity with mouse, rat, bovine, and canine CD42b. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves CD42b, between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). CD42b binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of CD42b to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin  alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in CD42b (1, 14).

References
  1. Andrews, R.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1511. 
  2. Lopez, J.A. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5615.
  3. Wenger, R.H. et al. (1988) Biochem. Biophys. Res. Commun. 156:389. 
  4. Luo, S-Z. et al. (2007) Blood 109:603. 
  5. Uff, S. et al. (2002) J. Biol. Chem. 277:35657.
  6. Shen, Y. et al. (2006) J. Biol. Chem. 281:26419.
  7. Ishida, F. et al. (1995) Blood 86:1356.
  8. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
  9. Beer, J.H. et al. (1994) Blood 83:691.
  10. Adam, F. et al. (2003) Eur. J. Biochem. 270:2959.
  11. Baglia, F.A. et al. (2004) J. Biol. Chem. 279:49323.
  12. Bradford, H.N. et al. (2000) J. Biol. Chem. 275:22756.
  13. Wang, Y. et al. (2005) Circulation 112:2993.
  14. Othman, M. et al. (2005) Blood 105:4330.
Long Name
Glycoprotein lb [Platelet] alpha
Entrez Gene IDs
2811 (Human); 14723 (Mouse)
Alternate Names
Antigen CD42b-alpha; BP1BA; BSS; CD42b antigen; CD42b; CD42b-alpha; glycoprotein Ib (platelet), alpha polypeptide; Glycoprotein Ibalpha; GP1B; GP1BA; GPIb alpha; GP-Ib alpha; GPIbA; GPIb-alpha; MGC34595; platelet glycoprotein Ib alpha chain; platelet membrane glycoprotein 1b-alpha subunit

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