Human CD42b/GPIb alpha Antibody Summary
Accession # P07359
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CD42b/GPIb alpha by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD42b/GPIb alpha in Human CD41+Platelets by Flow Cytometry. Human CD41+platelets were stained with Mouse Anti-Human Integrin a2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD42b/GPIb alpha
Platelet glycoprotein Ib alpha chain (GPIb alpha ), also known as CD42b, is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human CD42b contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature CD42b are known to exist but have unknown significance (7). The ECD of human CD42b shares 48‑51% aa identity with mouse, rat, bovine, and canine CD42b. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves CD42b, between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). CD42b binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of CD42b to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in CD42b (1, 14).
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- Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
- Beer, J.H. et al. (1994) Blood 83:691.
- Adam, F. et al. (2003) Eur. J. Biochem. 270:2959.
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- Wang, Y. et al. (2005) Circulation 112:2993.
- Othman, M. et al. (2005) Blood 105:4330.
Citation for Human CD42b/GPIb alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Nanoparticle biointerfacing by platelet membrane cloaking.
Authors: Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen C, Kroll A, Carpenter C, Ramesh M, Qu V, Patel S, Zhu J, Shi W, Hofman F, Chen T, Gao W, Zhang K, Chien S, Zhang L
Sample Types: Cell Lysates
Applications: Western Blot
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