Detection of Human CD42b/GPIb alpha by Western Blot.
Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD42b/GPIb alpha Monoclonal Antibody (Catalog # MAB4067) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD42b/GPIb alpha at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD42b/GPIb alpha in Human CD41+ Platelets by Flow Cytometry.
Human CD41+ platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human CD42b/GPIb alpha Monoclonal Antibody (Catalog # MAB4067) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD42b/GPIb alpha
Platelet glycoprotein Ib alpha chain (GPIb alpha ), also known as CD42b, is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human CD42b contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature CD42b are known to exist but have unknown significance (7). The ECD of human CD42b shares 48‑51% aa identity with mouse, rat, bovine, and canine CD42b. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves CD42b, between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). CD42b binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of CD42b to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in CD42b (1, 14).
Andrews, R.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1511.
Lopez, J.A. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5615.
Wenger, R.H. et al. (1988) Biochem. Biophys. Res. Commun. 156:389.
Luo, S-Z. et al. (2007) Blood 109:603.
Uff, S. et al. (2002) J. Biol. Chem. 277:35657.
Shen, Y. et al. (2006) J. Biol. Chem. 281:26419.
Ishida, F. et al. (1995) Blood 86:1356.
Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
Beer, J.H. et al. (1994) Blood 83:691.
Adam, F. et al. (2003) Eur. J. Biochem. 270:2959.
Baglia, F.A. et al. (2004) J. Biol. Chem. 279:49323.
Bradford, H.N. et al. (2000) J. Biol. Chem. 275:22756.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.