Human CD47 Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD47

CD47 (also integrin-associated protein/IAP and OA3) is a variably glycosylated, 40‑60 kDa atypical member of the Ig-Superfamily. It is expressed on almost all cell types, including erythrocytes. CD47 binds to TSP-1 and SIRP alpha, and forms a membrane complex with CD36 and  alpha _v beta ₃. Mature human CD47 is a 305 amino acid (aa), five-transmembrane glycoprotein. It contains a 123 aa extracellular region (aa 19‑141) that is characterized by the presence of a V-type Ig-like domain (aa 19‑127), and a 34 aa C-terminal cytoplasmic tail that interacts with Gi alpha subunits. Three splice variants occur over aa 293‑323. Over aa 19‑139, human CD47 shares 61%, 71% and 66% aa identity with mouse, porcine and canine CD47, respectively.

Product Datasheets

Related Research Areas

• Acute Myeloid Leukemia
• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on VSMC
• Cancer Stem Cell Markers
• Dendritic Cell Pathogen Recognition/Uptake
• Immune Regulatory Molecules on Endothelial Cells
• Immunoglobulin Superfamily CAMs
• Integrins and Associated Molecules
• Lymphoma
• Microglia Activation
• Microglia Homeostasis
• Search Immune Checkpoint Targets
• Uptake of Amyloid beta by Glia Cells