Human CD47 Antibody

Catalog # Availability Size / Price Qty
AF4670
AF4670-SP
Human CD47 Antibody in Western Blot
5 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human CD47 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD47 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD47 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
CD47 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in CD47 knockout HEK293T cell line.
 
Neutralization
Measured by its ability to neutralize SIRP alpha /CD172a-mediated adhesion of human erythrocytes.  The adhesion of human erythrocytes to immobilized Recombinant Human SIRP alpha /CD172a/Fc Chimera (2 µg/mL, 100 µL/well) was maximally inhibited (70-100%) by 2 µg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CD47 by Western Blot. View Larger

Detection of Human CD47 by Western Blot. fWestern blot shows lysates of U937 human histiocytic lymphoma cell line and human placenta tissue, not heated to minimize aggregation. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD47 at approximately 45‑70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD47 in Human Lymphocytes by Flow Cytometry. View Larger

Detection of CD47 in Human Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Sheep Anti-Human CD47 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4670, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunohistochemistry CD47 in Human Placenta. View Larger

CD47 in Human Placenta. CD47 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4670) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Adhesion Mediated by SIRP alpha /CD172a and Neutral­ization by Human CD47 Antibody. View Larger

Cell Adhesion Mediated by SIRP alpha /CD172a and Neutral­ization by Human CD47 Antibody. Recombinant Human SIRP alpha /CD172a (Catalog # 4546-SA), immobilized onto a microplate, supports the adhesion of the human erythrocytes in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human SIRP-alpha (2 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human CD47 Poly­clonal Antibody (Catalog # AF4670). The adhesion was maximally inhibited (70-100%) by 2 µg/mL of the antibody.

Knockout Validated Western  Blot Shows Human CD47 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human CD47 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CD47 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD47 at approximately 50 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockoutHEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD47

CD47 (also integrin-associated protein/IAP and OA3) is a variably glycosylated, 40‑60 kDa atypical member of the Ig-Superfamily. It is expressed on almost all cell types, including erythrocytes. CD47 binds to TSP-1 and SIRP alpha, and forms a membrane complex with CD36 and  alpha v beta 3. Mature human CD47 is a 305 amino acid (aa), five-transmembrane glycoprotein. It contains a 123 aa extracellular region (aa 19‑141) that is characterized by the presence of a V-type Ig-like domain (aa 19‑127), and a 34 aa C-terminal cytoplasmic tail that interacts with Gi alpha subunits. Three splice variants occur over aa 293‑323. Over aa 19‑139, human CD47 shares 61%, 71% and 66% aa identity with mouse, porcine and canine CD47, respectively.

Entrez Gene IDs
961 (Human); 16423 (Mouse); 102139846 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

Product Datasheets

Citations for Human CD47 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma
    Authors: JH Wang, ST Huang, L Zhang, ZG Liu, RX Liang, SW Jiang, YN Jiang, XJ Yu, YC Jiang, XZ Li, PF Zhang, ZS Wen, M Zheng
    Cancer Med, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment
    Authors: Koichi Sakakura
    Lab Invest, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
    Authors: Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bauerle T, Wallwiener M, Holland-Letz T, Hofner T, Sprick M, Scharpff M, Marme F, Sinn H, Pantel K, Weichert W, Trumpp A
    Nat Biotechnol, 2013;31(6):539-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD47 Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CD47 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CD47 Antibody
By Anonymous on 05/20/2019
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human

Human CD47 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: Breast cancer cells Species: Human

Human CD47 Antibody
By Anonymous on 07/15/2018
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human