Human CD79B Alexa Fluor® 700-conjugated Antibody

Catalog #: AF6620N Datasheet / COA / SDS

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AF6620N-100UG

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Human CD79B Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD79B in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD79B is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster cell line CHO-derived recombinant human CD79B
Ala29-Asp159
Accession # P40259

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD79B

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta -chain) is a 36-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with 44-49 kDa CD79A. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. Mature human CD79B is a 201 amino acid (aa) type I tranamembrane glycoprotein (aa 29‑229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 181-229). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation. Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.

Entrez Gene IDs

974 (Human); 15985 (Mouse); 171055 (Rat); 102145117 (Cynomolgus Monkey)

Alternate Names

B29; B29B-cell-specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; CD79B antigen (immunoglobulin-associated beta); CD79b antigen; CD79b molecule, immunoglobulin-associated beta; CD79B; IGB; IGBAGM6; Ig-beta; Immunoglobulin-associated B29 protein