Human CD79B Antibody Summary
Accession # P40259
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD79B in Human peripheral blood lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Sheep Anti-Human CD79B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6620) followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
Detection of Human CD79B by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD79B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6620) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79B at approximately 40kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta -chain) is a 36-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with 44-49 kDa CD79A. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. Mature human CD79B is a 201 amino acid (aa) type I tranamembrane glycoprotein (aa 29‑229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 181-229). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation. Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.
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