Detection of CD8 alpha in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD8 alpha Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1509V) or (B) Mouse IgG2B Alexa Fluor 405 Isotype Control (Catalog # IC0041V). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD8 is a dimeric glycoprotein consisting of either two alpha chains or an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC-I/peptide complexes.
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