Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry. |
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1509G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
CD8 alpha in Human PBMCs. |
CD8 alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1509G; green) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.