Human CD8 alpha Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1509G-100
FAB1509G-025
Human CD8 alpha  Alexa Fluor® 488-conjugated Antibody in Data
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Human CD8 alpha Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.
Source
Monoclonal Mouse IgG2B Clone # 37006
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741969
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD8 alpha  in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1509G) or (B) Mouse IgG2B isotype control antibody (Catalog # IC003G) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD8 alpha  in Human PBMCs. View Larger

CD8 alpha in Human PBMCs. CD8 alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1509G; green) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Human CD8 alpha Alexa Fluor® 488-conjugated Antibody
By Anonymous on 03/21/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human