Human CD8 alpha Alexa Fluor® 488-conjugated Antibody Summary
Accession # NP_741969
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1509G) or (B) Mouse IgG2B isotype control antibody (Catalog # IC003G) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.
CD8 alpha in Human PBMCs. CD8 alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1509G; green) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.
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