Human CDCP1 Antibody [Biotin]

R&D Systems | Catalog # BAF2666

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CDCP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Phe30-Leu666
Accession # NP_073753

Specificity

Detects human CDCP1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CDCP1 Antibody [Biotin]

Detection of CDCP1 in SW480 cells by Flow Cytometry

SW480 cells were stained with Goat Anti-Human CDCP1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2666, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CDCP1 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: SW480 human colorectal adenocarcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human CDCP1

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 amino acid (aa) extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa sequence identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

NP_073753

Additional CDCP1 Products

Product Documents for Human CDCP1 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CDCP1 Antibody [Biotin]

For research use only

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