Human CDCP1 Antibody Summary
Accession # NP_073753
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CDCP1 in Human Colon Cancer Tissue. CDCP1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.
Citations for Human CDCP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
Authors: Kelly OG, Chan MY, Martinson LA
Nat. Biotechnol., 2011;29(8):750-6.
Sample Types: Whole Tissue
Proteolysis-induced N-terminal ectodomain shedding of the integral membrane glycoprotein CUB domain-containing protein 1 (CDCP1) is accompanied by tyrosine phosphorylation of its C-terminal domain and recruitment of Src and PKCdelta.
Authors: He Y, Wortmann A, Burke LJ
J. Biol. Chem., 2010;285(34):26162-73.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human CDCP1 Antibody
There are currently no reviews for this product. Be the first to review Human CDCP1 Antibody and earn rewards!
Have you used Human CDCP1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image