Human Cerebellin-4 Antibody Summary
Accession # Q9NTU7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cerebellin-4 in Human Hypothalamus. Cerebellin-4 was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Sheep Anti-Human Cerebellin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6740) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CBLN4 (Cerebellin-4) is a 26-35 kDa, secreted glycoprotein member of the C1q/TNF Superfamily of molecules. It is expressed in developing Sertoli cells and select neurons in the dorsal raphe, entorhinal cortex and arcuate nucleus. In the testis, it likely promotes cell differentiation; in the brain, it is possible that it participates in synapse formation via an interaction with neurexin. Mature human CBLN4 is 174 amino acids (aa) in length (aa 28-201). It contains two N-terminal cysteines that mediate homohexamer formation, and a C-terminal C1q domain (aa 66-201) that promotes homotrimer formation. CBLN4 also reportedly has the potential to multimerize with the other CBLN-related molecules, CBLN1-3. Mature human CBLN4 shares 99% aa identity with mouse CBLN4.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human Cerebellin-4 Antibody
There are currently no reviews for this product. Be the first to review Human Cerebellin-4 Antibody and earn rewards!
Have you used Human Cerebellin-4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image