Human CFTR C-Terminus Antibody

Catalog # Availability Size / Price Qty
MAB25031
MAB25031-SP
Human CFTR C-Terminus Antibody in Data
2 Images
Product Details
Citations (42)
FAQs
Supplemental Products
Reviews (1)

Human CFTR C-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 24-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Glutathione S-transferase-coupled CFTR
aa 1377-1480
Accession # P13569
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human CFTR transfected cell line
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
1-2 µg/106 cells
T84 human colon carcinoma cell line, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CFTR antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CFTR antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CFTR

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Long Name
Cystic Fibrosis Transmembrane Conductance Regulator
Entrez Gene IDs
1080 (Human)
Alternate Names
ABC35; ABCC7CF; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7); cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7); dJ760C5.1; EC 3.6.3; MRP7EC 3.6.3.49; TNR-CFTR

Product Datasheets

Citations for Human CFTR C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium
    Authors: M Badaoui, A Zoso, T Idris, M Bacchetta, J Simonin, S Lemeille, B Wehrle-Hal, M Chanson
    Cell Rep, 2020;32(1):107842.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Treatment of Cystic Fibrosis Patients Homozygous for F508del with Lumacaftor-Ivacaftor (Orkambi�) Restores Defective CFTR Channel Function in Circulating Mononuclear Cells
    Authors: M Favia, C Gallo, L Guerra, D De Venuto, A Diana, AM Polizzi, P Montemurro, MA Mariggiò, G Leonetti, A Manca, V Casavola, M Conese
    Int J Mol Sci, 2020;21(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation
    Authors: G Ortiz-Muno, MA Yu, E Lefrançais, B Mallavia, C Valet, JJ Tian, S Ranucci, KM Wang, Z Liu, N Kwaan, D Dawson, ME Kleinhenz, FT Khasawneh, PM Haggie, AS Verkman, MR Looney
    J. Clin. Invest., 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation
    Authors: S Malakpour, J Coimbra, JM Wilson
    PLoS ONE, 2018;13(10):e0206206.
    Species: Fish - Plotosus lineatus (Striped Eel Catfish)
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene
    Authors: D De Rocco, B Pompili, S Castellani, E Morini, L Cavinato, G Cimino, MA Mariggiò, S Guarnieri, M Conese, P Del Porto, F Ascenzioni
    Int J Mol Sci, 2018;19(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Synergy of cAMP and calcium signaling pathways in CFTR regulation
    Authors: Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
  7. Ammonia exposure affects the mRNA and protein expression levels of certain Rhesus glycoproteins in the gills of climbing perch
    Authors: XL Chen, B Zhang, YR Chng, JLY Ong, SF Chew, WP Wong, SH Lam, T Nakada, YK Ip
    J. Exp. Biol., 2017;0(0):.
    Species: Fish
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling
    Authors: A Schögler, F Blank, M Brügger, S Beyeler, SA Tschanz, N Regamey, C Casaulta, T Geiser, MP Alves
    Respir. Res., 2017;18(1):215.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR
    Authors: MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M
    Physiol Rep, 2017;5(13):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages
    Authors: CL Shrestha, KD Assani, H Rinehardt, F Albastroiu, S Zhang, R Shell, AO Amer, LS Schlesinge, BT Kopp
    PLoS ONE, 2017;12(10):e0186169.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Deep interactome profiling of membrane proteins by co-interacting protein identification technology
    Nat Protoc, 2016;11(12):2515-2528.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  12. New use for an old drug: COX-independent anti-inflammatory effects of sulindac in models of cystic fibrosis
    Authors: Agathe Tarze
    Br. J. Pharmacol., 2016;173(11):1728-41.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Western Blot
  13. CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction.
    Authors: Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W
    Proc Natl Acad Sci U S A, 2015;112(13):E1614-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.
    Authors: Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V
    PLoS ONE, 2015;10(3):e0118943.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water.
    Authors: Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S
    PLoS ONE, 2014;9(1):e87591.
    Species: Fish
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR).
    Authors: Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H
    J Biol Chem, 2014;289(52):35953-68.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  17. Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells.
    Authors: Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M
    J Cell Mol Med, 2014;18(8):1631-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
    Authors: Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M
    J Immunol, 2014;192(10):4804-12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels.
    Authors: Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E
    Respir Res, 2014;15(0):69.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Correcting the cystic fibrosis disease mutant, A455E CFTR.
    Authors: Cebotaru L, Rapino D, Cebotaru V, Guggino W
    PLoS ONE, 2014;9(1):e85183.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells.
    Authors: Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M
    PLoS ONE, 2013;8(4):e60211.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  22. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
    Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
    J Biol Chem, 2013;288(43):31177-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  23. Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium.
    Authors: Oglesby I, Chotirmall S, McElvaney N, Greene C
    J Immunol, 2013;190(7):3354-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.
    Authors: Duan, Yuanyuan, Sun, Ying, Zhang, Fan, Zhang, Wei Kevi, Wang, Dong, Wang, Yan, Cao, Xu, Hu, Wenbao, Xie, Changyan, Cuppoletti, John, Magin, Thomas M, Wang, Haixia, Wu, Zhenguo, Li, Ning, Huang, Pingbo
    J Biol Chem, 2012;287(48):40547-59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  25. Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.
    Authors: Murphy, Sean V, Lim, Rebecca, Heraud, Philip, Cholewa, Marian, Le Gros, Mark, de Jonge, Martin D, Howard, Daryl L, Paterson, David, McDonald, Courtney, Atala, Anthony, Jenkin, Graham, Wallace, Euan M
    PLoS ONE, 2012;7(9):e46533.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein.
    Authors: Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C
    PLoS ONE, 2012;7(6):e40173.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  27. Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase.
    Authors: King, J Darwin, Lee, Jeffrey, Riemen, Claudia, Neumann, Dietbert, Xiong, Sheng, Foskett, J Kevin, Mehta, Anil, Muimo, Richmond, Hallows, Kenneth
    J Biol Chem, 2012;287(40):33389-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  28. Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.
    Authors: Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P
    PLoS ONE, 2011;6(7):e22212.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
    Authors: Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
    Am. J. Pathol., 2009;174(3):869-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing.
    Authors: Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB
    J. Biol. Chem., 2008;283(32):21926-33.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo.
    Authors: Tang JY, Au DW
    Biochem. Biophys. Res. Commun., 2007;353(1):98-103.
    Species: Fish - Goldline seabream
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones.
    Authors: Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ
    J. Endocrinol., 2007;192(1):249-60.
    Species: Fish
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone.
    Authors: Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE
    Thorax, 2007;62(7):650-1.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries.
    Authors: Robert R, Savineau JP, Norez C, Becq F, Guibert C
    Eur. Respir. J., 2007;30(5):857-64.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  35. Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
    Authors: Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):72-83.
    Species: Ovine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis.
    Authors: Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G
    Biochemistry, 2006;45(34):10260-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells.
    Authors: Nanua S, Sajjan U, Keshavjee S, Hershenson MB
    Biochem. Biophys. Res. Commun., 2006;343(1):135-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype.
    Authors: Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P
    Hum. Mutat., 2005;25(4):360-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  39. Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
    Authors: Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
    J. Cell. Sci., 2004;117(0):1923-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  40. Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models.
    Authors: Pan J, Bear C, Farragher S, Cutz E, Yeger H
    Am. J. Respir. Cell Mol. Biol., 2002;27(5):553-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
    Authors: Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH
    Biochim. Biophys. Acta, 2002;1566(1):28-43.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-P
  42. Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.
    Authors: Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
    J. Biol. Chem., 2002;277(24):21480-8.
    Species: Amphibian
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CFTR C-Terminus Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CFTR C-Terminus Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CFTR C-Terminus Antibody
By Anonymous on 06/29/2016
Application: ICC/IF Sample Tested: Intestine,gills Species: Gulf toadfish,Killifish

Also used in Western blots. The dilution factor for the Western blots was 1:50, so it's not the most specific antibody against fish CFTR, however, the banding was clear and the immunohistochemistry came out adequately.