Human CFTR C-Terminus Antibody

(33 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 24-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Glutathione S-transferase-coupled CFTR
    aa 1377-1480
    Accession # P13569
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CFTR
Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).
  • Long Name:
    Cystic Fibrosis Transmembrane Conductance Regulator
  • Entrez Gene IDs:
    1080 (Human)
  • Alternate Names:
    ABC35; ABCC7CF; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7); cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7); dJ760C5.1; EC 3.6.3; MRP7EC 3.6.3.49; TNR-CFTR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR
    Authors: MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M
    Physiol Rep, 2017;5(13):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  2. Synergy of cAMP and calcium signaling pathways in CFTR regulation
    Authors: Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
  3. Deep interactome profiling of membrane proteins by co-interacting protein identification technology
    Nat Protoc, 2016;11(12):2515-2528.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  4. CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction.
    Authors: Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W
    Proc Natl Acad Sci U S A, 2015;112(13):E1614-23.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  5. Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.
    Authors: Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V
    PLoS ONE, 2015;10(3):e0118943.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR).
    Authors: Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H
    J Biol Chem, 2014;289(52):35953-68.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  7. Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
    Authors: Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M
    J Immunol, 2014;192(10):4804-12.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells.
    Authors: Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M
    J Cell Mol Med, 2014;18(8):1631-43.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels.
    Authors: Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E
    Respir Res, 2014;15(0):69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water.
    Authors: Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S
    PLoS ONE, 2014;9(1):e87591.
    Species: Fish
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Correcting the cystic fibrosis disease mutant, A455E CFTR.
    Authors: Cebotaru L, Rapino D, Cebotaru V, Guggino W
    PLoS ONE, 2014;9(1):e85183.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Cell Lysates
    Application: WB
  12. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
    Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
    J Biol Chem, 2013;288(43):31177-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  13. Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium.
    Authors: Oglesby I, Chotirmall S, McElvaney N, Greene C
    J Immunol, 2013;190(7):3354-62.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells.
    Authors: Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M
    PLoS ONE, 2013;8(4):e60211.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  15. Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.
    Authors: Murphy, Sean V, Lim, Rebecca, Heraud, Philip, Cholewa, Marian, Le Gros, Mark, de Jonge, Martin D, Howard, Daryl L, Paterson, David, McDonald, Courtney, Atala, Anthony, Jenkin, Graham, Wallace, Euan M
    PLoS ONE, 2012;7(9):e46533.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase.
    Authors: King, J Darwin, Lee, Jeffrey, Riemen, Claudia, Neumann, Dietbert, Xiong, Sheng, Foskett, J Kevin, Mehta, Anil, Muimo, Richmond, Hallows, Kenneth
    J Biol Chem, 2012;287(40):33389-400.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  17. Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.
    Authors: Duan, Yuanyuan, Sun, Ying, Zhang, Fan, Zhang, Wei Kevi, Wang, Dong, Wang, Yan, Cao, Xu, Hu, Wenbao, Xie, Changyan, Cuppoletti, John, Magin, Thomas M, Wang, Haixia, Wu, Zhenguo, Li, Ning, Huang, Pingbo
    J Biol Chem, 2012;287(48):40547-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  18. Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein.
    Authors: Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C
    PLoS ONE, 2012;7(6):e40173.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  19. Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.
    Authors: Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P
    PLoS ONE, 2011;6(7):e22212.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
    Authors: Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
    Am. J. Pathol., 2009;174(3):869-80.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  21. Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing.
    Authors: Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB
    J. Biol. Chem., 2008;283(32):21926-33.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones.
    Authors: Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ
    J. Endocrinol., 2007;192(1):249-60.
    Species: Fish
    Sample Type: Tissue Homogenates
    Application: WB
  23. Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone.
    Authors: Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE
    Thorax, 2007;62(7):650-1.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  24. Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries.
    Authors: Robert R, Savineau JP, Norez C, Becq F, Guibert C
    Eur. Respir. J., 2007;30(5):857-64.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
  25. The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo.
    Authors: Tang JY, Au DW
    Biochem. Biophys. Res. Commun., 2007;353(1):98-103.
    Species: Fish - Goldline seabream
    Sample Type: Tissue Homogenates
    Application: WB
  26. CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis.
    Authors: Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G
    Biochemistry, 2006;45(34):10260-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  27. Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
    Authors: Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):72-83.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  28. Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells.
    Authors: Nanua S, Sajjan U, Keshavjee S, Hershenson MB
    Biochem. Biophys. Res. Commun., 2006;343(1):135-43.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  29. Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype.
    Authors: Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P
    Hum. Mutat., 2005;25(4):360-71.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  30. Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
    Authors: Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
    J. Cell. Sci., 2004;117(0):1923-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  31. Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
    Authors: Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH
    Biochim. Biophys. Acta, 2002;1566(1):28-43.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  32. Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models.
    Authors: Pan J, Bear C, Farragher S, Cutz E, Yeger H
    Am. J. Respir. Cell Mol. Biol., 2002;27(5):553-60.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  33. Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.
    Authors: Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
    J. Biol. Chem., 2002;277(24):21480-8.
    Species: Amphibian
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 33 Citations
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedIntestine,gills

Other Experimental Details

Other Experimental DetailsAlso used in Western blots. The dilution factor for the Western blots was 1:50, so it's not the most specific antibody against fish CFTR, however, the banding was clear and the immunohistochemistry came out adequately.

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