Clathrin Heavy Chain 1/CHC17 (clathrin heavy chain 17; also CHC, CLTCL2 and CLTC) is a 170‑180 kDa member of the clathrin heavy chain family of molecules. It is widely expressed, and serves at least two functions. First, it interacts with TACC3 and chTOG to form a complex that crosslinks microtubules making up K‑fibers, and second, three Clathrin Heavy Chain 1/CHC17 molecules form a triskelion (Greek for "3 legged") structure with three clathrin light chains to create the polyhedral coat of coated pits. Human Clathrin Heavy Chain 1/CHC17 is 1675 amino acids (aa) in length. It contains an N‑terminal domain that mediates protein‑protein interactions (aa 2‑479), a flexible linker region (aa 480‑523), a distal segment (aa 524‑638), a proximal region that interacts noncovalently with one CHC light chain (aa 639‑1549), and a trimerization domain that mediates the formation of a noncovalent triskelion structure (aa 1550‑1675). Although the predicted MW of Clathrin Heavy Chain 1/CHC17 is 191 kDa, it runs anomalously at 180 kDa in SDS‑PAGE. Multiple utilized phosphorylation and acetylation sites exist. There are multiple potential splice forms. One shows a four aa insertion after Lys83, a second shows a seven aa insertion after Asp1611, and a third contains a four aa substitution for aa 1636‑1675. Over aa 1521‑1675, human and mouse Clathrin Heavy Chain 1/CHC17 are identical in aa sequence.
Human Clathrin Heavy Chain 1/CHC17 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # AF6784V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Trp1521-Met1675
Accession # Q00610
Specificity
Clonality
Host
Isotype
Applications
Immunocytochemistry
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Clathrin Heavy Chain 1/CHC17
Long Name
Alternate Names
Gene Symbol
UniProt
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Protocols
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- Appropriate Fixation of IHC/ICC Samples
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- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
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- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
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- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
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- The Importance of IHC/ICC Controls
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- Western Blot Conditions
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- Western Blot Troubleshooting
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