Human Clathrin Heavy Chain 1/CHC17 Antibody

Catalog # Availability Size / Price Qty
AF6784
AF6784-SP
Detection of Human Clathrin Heavy Chain 1/CHC17 by Western Blot.
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Human Clathrin Heavy Chain 1/CHC17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Clathrin Heavy Chain 1/CHC17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC-22 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Clathrin Heavy Chain 1/CHC17
Trp1521-Met1675
Accession # Q00610
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Clathrin Heavy Chain 1/CHC17 by Western Blot. View Larger

Detection of Human Clathrin Heavy Chain 1/
CHC17 by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line and human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 1/CHC17 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Clathrin Heavy Chain 1/CHC17 in HeLa Human Cell Line. View Larger

Clathrin Heavy Chain 1/CHC17 in HeLa Human Cell Line. Clathrin Heavy Chain 1/CHC17 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Clathrin coated vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clathrin Heavy Chain 1/CHC17

Clathrin Heavy Chain 1/CHC17 (clathrin heavy chain 17; also CHC, CLTCL2 and CLTC) is a 170‑180 kDa member of the clathrin heavy chain family of molecules. It is widely expressed, and serves at least two functions. First, it interacts with TACC3 and chTOG to form a complex that crosslinks microtubules making up K‑fibers, and second, three Clathrin Heavy Chain 1/CHC17 molecules form a triskelion (Greek for "3 legged") structure with three clathrin light chains to create the polyhedral coat of coated pits. Human Clathrin Heavy Chain 1/CHC17 is 1675 amino acids (aa) in length. It contains an N‑terminal domain that mediates protein‑protein interactions (aa 2‑479), a flexible linker region (aa 480‑523), a distal segment (aa 524‑638), a proximal region that interacts noncovalently with one CHC light chain (aa 639‑1549), and a trimerization domain that mediates the formation of a noncovalent triskelion structure (aa 1550‑1675). Although the predicted MW of Clathrin Heavy Chain 1/CHC17 is 191 kDa, it runs anomalously at 180 kDa in SDS‑PAGE. Multiple utilized phosphorylation and acetylation sites exist. There are multiple potential splice forms. One shows a four aa insertion after Lys83, a second shows a seven aa insertion after Asp1611, and a third contains a four aa substitution for aa 1636‑1675. Over aa 1521‑1675, human and mouse Clathrin Heavy Chain 1/CHC17 are identical in aa sequence.

Long Name
Clathrin Heavy Chain on Chromosome 17
Entrez Gene IDs
1213 (Human); 67300 (Mouse); 54241 (Rat)
Alternate Names
CHC; CHC17; Clathrin Heavy Chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy chain; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLTC; CLTCL2; Hc; KIAA0034

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