Human CLEC-2/CLEC1B APC-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CLEC-2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human CLEC‑1.
  • Source
    Monoclonal Mouse IgG2A Clone # 219133
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0-derived rhCLEC-2 extracellular domain
    Gln58-Pro229
    Accession # AAF36777
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CLEC‑2 in Human Whole Blood by Flow Cytometry. Human whole blood was stained with Mouse Anti-Human CD41 FITC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human CLEC‑2 APC‑conjugated Monoclonal Antibody (Catalog # FAB1718A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CLEC-2/CLEC1B
C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the platelet-aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).
  • References:
    1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
    2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
    3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
    4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
    5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
    6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
    7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
    8. Chaipan, C. et al. (2006) J. Virol. 80:8951.
  • Long Name:
    C-type Lectin-like Receptor 2
  • Entrez Gene IDs:
    51266 (Human); 56760 (Mouse)
  • Alternate Names:
    1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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