Human CLEC-2/CLEC1B APC‑conjugated Antibody

R&D Systems | Catalog # FAB1718A

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 219133
View all CLEC-2/CLEC1B Primary Antibodies »

Product Specifications

Immunogen

NS0-derived rhCLEC-2 extracellular domain
Gln58-Pro229
Accession # AAF36777

Specificity

Detects human CLEC-2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human CLEC‑1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CLEC-2/CLEC1B APC‑conjugated Antibody

Detection of CLEC-2 antibody in Human Whole Blood antibody by Flow Cytometry.

Detection of CLEC‑2 in Human Whole Blood by Flow Cytometry.

Human whole blood was stained with Mouse Anti-Human CD41 FITC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human CLEC-2 APC-conjugated Monoclonal Antibody (Catalog # FAB1718A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Detection of CLEC-2/CLEC1B in platelets by Flow Cytometry.

Platelets were stained with Mouse Anti-Human Integrin  alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human CLEC-2/CLEC1B APC‑conjugated Monoclonal Antibody (Catalog # FAB1718A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CLEC-2/CLEC1B APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human whole blood, platelets

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the platelet-aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

References

  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Long Name

C-type Lectin-like Receptor 2

Alternate Names

CLEC1B, CLEC2

Entrez Gene IDs

51266 (Human); 56760 (Mouse)

Gene Symbol

CLEC1B

UniProt

AAF36777

Additional CLEC-2/CLEC1B Products

Product Documents for Human CLEC-2/CLEC1B APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CLEC-2/CLEC1B APC‑conjugated Antibody

For research use only

Citations for Human CLEC-2/CLEC1B APC‑conjugated Antibody

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