Human CLEC-2/CLEC1B Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Human CLEC-2/CLEC1B Biotinylated Antibody Summary

Species Reactivity
Detects human CLEC-2/CLEC1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CLEC-1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human CLEC-2/CLEC1B
Accession # AAF36777
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human CLEC-2/CLEC1B (Catalog # 1718-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.
Long Name
C-type Lectin-like Receptor 2
Entrez Gene IDs
51266 (Human); 56760 (Mouse)
Alternate Names
1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CLEC-2/CLEC1B Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human CLEC-2/CLEC1B Biotinylated Antibody and earn rewards!

Have you used Human CLEC-2/CLEC1B Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review