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Human CLEC-2A Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB72191R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB72191R-100UG

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Human CLEC-2A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC-2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.
Source
Monoclonal Mouse IgG1 Clone # 705529
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-2A
Trp49-Leu174 (predicted)
Accession # Q6UVW9
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CLEC-2A

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8+ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

Long Name
C-type Lectin Domain Family 2, Member A
Entrez Gene IDs
387836 (Human)
Alternate Names
CLEC2A C-type lectin domain family 2, member A ; CLEC2A; CLEC-2A; KACL; PILAR; UNQ5792

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