Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 705529
View all CLEC-2A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC-2A
Trp49-Leu174 (predicted)
Accession # Q6UVW9

Specificity

Detects human CLEC-2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CLEC-2A Antibody

Detection of Human CLEC-2A antibody by Western Blot.

Detection of Human CLEC-2A by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CLEC-2A antibody in Human Skin by Immunohistochemistry (IHC-P).

CLEC-2A in Human Skin.

CLEC-2A was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CLEC-2A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC-2A

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8+ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

Long Name

C-type Lectin Domain Family 2, Member A

Alternate Names

CLEC2A, KACL, PILAR

Entrez Gene IDs

387836 (Human)

Gene Symbol

CLEC2A

UniProt

Q6UVW9 (Human)

Additional CLEC-2A Products

Product Documents for Human CLEC-2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CLEC-2A Antibody

For research use only

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Protocols

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