Human CLEC3B/Tetranectin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CLEC3B/Tetranectin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 805035
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CLEC3B/Tetranectin
    Glu22-Val202
    Accession # P05452
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction

    In a functional ELISA, 5-25 μg/mL of this antibody will block 50% of the binding of 10 μg/mL of Recombinant Human CLEC3B to immobilized Recombinant Human HGF coated at 5 μg/mL (100 μL/well). At 50 μg/mL, this antibody will block >90% of the binding

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CLEC3B/Tetranectin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CLEC3B/Tetranectin by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC3B/Tetranectin

CLEC3B (C-type lectin domain family 3-member B; also Tetranectin) is a 20 kDa, secreted member of the C type lectin superfamily. It is produced by multiple cell types including monocytes, neutrophils, fibroblasts, hepatocytes and various endocrine cells. Although named Tetranectin, CLEC3B is actually a nondisulfide linked homotrimer. It binds to plasminogen, sulfated polysaccharides, and fibrin. Human CLEC3B precursor is 202 amino acids (aa) in length. It contains a signal sequence (aa 1-21), a coiled coil region that mediates trimerization (aa 26-52) and a C type lectin domain (aa 77-198). CLEC3B contains O-linked glycosylation. Mature human CLEC3B is 81% aa identical to mouse CLEC3B.

  • Long Name:
    C-type Lectin-like Receptor 3B
  • Entrez Gene IDs:
    7123 (Human); 21922 (Mouse)
  • Alternate Names:
    C-type lectin domain family 3 member B; C-type lectin domain family 3, member B; DKFZp686H17246; Plasminogen kringle 4-binding protein; TETN; tetranectin (plasminogen binding protein); Tetranectin; TNA; TNAtetranectin (plasminogen-binding protein); TNtetranectin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 21  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0042 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL007 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Antibody

WB AF007 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CLEC3B/Tetranectin Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators
Reconstitution Calculator
=
÷
Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Feedback

Customer Information