CLEC3B (C-type lectin domain family 3-member B; also Tetranectin) is a 20 kDa, secreted member of the C type lectin superfamily. It is produced by multiple cell types including monocytes, neutrophils, fibroblasts, hepatocytes and various endocrine cells. Although named Tetranectin, CLEC3B is actually a nondisulfide linked homotrimer. It binds to plasminogen, sulfated polysaccharides, and fibrin. Human CLEC3B precursor is 202 amino acids (aa) in length. It contains a signal sequence (aa 1-21), a coiled coil region that mediates trimerization (aa 26-52) and a C type lectin domain (aa 77-198). CLEC3B contains O-linked glycosylation. Mature human CLEC3B is 81% aa identical to mouse CLEC3B.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Blockade of Receptor-ligand Interaction, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 805035
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC3B/Tetranectin
Glu22-Val202
Accession # P05452
Glu22-Val202
Accession # P05452
Specificity
Detects human CLEC3B/Tetranectin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CLEC3B/Tetranectin Antibody
Detection of Human CLEC3B/Tetranectin by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CLEC3B/Tetranectin by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human CLEC3B/Tetranectin Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
5 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
0.5 µg/mL
Sample: Human plasma
Sample: Human plasma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CLEC3B/Tetranectin
Long Name
C-type Lectin-like Receptor 3B
Alternate Names
TETN, Tetranectin, TNA
Gene Symbol
CLEC3B
UniProt
Additional CLEC3B/Tetranectin Products
Product Documents for Human CLEC3B/Tetranectin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CLEC3B/Tetranectin Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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