Human CLOCK Antibody Summary
Accession # O15516
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CLOCK by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Human CLOCK Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4026) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CLOCK at approximately 95 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CLOCK is an essential regulator of circadian rhythms. CLOCK is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for CLOCK’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human CLOCK Antibody
There are currently no reviews for this product. Be the first to review Human CLOCK Antibody and earn rewards!
Have you used Human CLOCK Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image